The overall aim of this WP is to develop robust and rapid sample preparations for the subsequent molecular detection of virus, bacteria and protozoa in large volumes of water. More precisely the objectives are:
- To improve pathogen extraction from concentrates obtained by using low-cost hollow fibre 2. To develop efficient pathogen concentration and extraction from filtrates obtained using nanotechnology-based filters.
- To optimise and assess the concentration and extraction of pathogens using ultra-low cost glass wool filteringtechnique.
- To develop and assess the concentration of pathogens and removal of inhibitors using novel microfluidicstructures.
- To evaluate and compare the recovery efficiency, sensitivity, quantifiability and inhibitor removal of the abovementioned techniques via the use of (RT-)PCR techniques.
- To address the issue of the diversity of water samples via an exchange of technologies between theparticipants across Europe.