Objectives
The overall aim of this WP is to develop robust and rapid sample preparations for the subsequent molecular detection of virus, bacteria and protozoa in large volumes of water. More precisely the objectives are:
- To improve pathogen extraction from concentrates obtained by using low-cost hollow fibre 2. To develop efficient pathogen concentration and extraction from filtrates obtained using nanotechnology-based filters.
- To optimise and assess the concentration and extraction of pathogens using ultra-low cost glass wool filteringtechnique.
- To develop and assess the concentration of pathogens and removal of inhibitors using novel microfluidicstructures.
- To evaluate and compare the recovery efficiency, sensitivity, quantifiability and inhibitor removal of the abovementioned techniques via the use of (RT-)PCR techniques.
- To address the issue of the diversity of water samples via an exchange of technologies between theparticipants across Europe.
Del 6.1 Review: Filtering, separation and purification techniques for molecular pathogen detection
Del 6.2 High-yield on-site pathogen concentration of 20-100L of different types of water
Del 6.3 Procedure to extract high quality nucleic acids from water concentrates for pathogen detection
Del 6.4 An evaluation of microfluidic systems to perform sample separation